Mice: check. Lizards: check. Squid: check. Marsupials … check.
CRISPR has been used to modify the genes of tomatoes, humans, and just about everything in between. Because of their unique reproductive biology and their relative rarity in laboratory settings, though, marsupials had eluded the CRISPR rush—until now.
A team of researchers at Japan’s Riken Institute, a national research facility, have used the technology to edit the genes of a South American species of opossum. The results were described in a new study out today in Current Biology. The ability to tweak marsupial genomes could help biologists learn more about the animals and use them to study immune responses, developmental biology, and even diseases like melanoma.
“I’m very excited to see this paper. It’s an accomplishment that I didn’t think would perhaps happen in my lifetime,” says John VandeBerg, a geneticist at the University of Texas Rio Grande Valley, who was not involved in the study.
The difficulties of genetically modifying marsupials had less to do with CRISPR than with the intricacies of marsupial reproductive biology, says Hiroshi Kiyonari (link in Japanese), the lead author of the new study.
While kangaroos and koalas are more well-known, researchers who study marsupials often use opossums in lab experiments, since they’re smaller and easier to care for. Gray short-tailed opossums, the species used in the study, are related to the white-faced North American opossums, but they’re smaller and don’t have a pouch.
The researchers at Riken used CRISPR to delete, or knock out, a gene that codes for pigment production. Targeting this gene meant that if the experiments worked, the results would be obvious at a glance: the opossums would be albino if both copies of the gene were knocked out, and mottled, or mosaic, if a single copy was deleted.
The resulting litter included one albino opossum and one mosaic opossum (pictured above). The researchers also bred the two, which resulted in a litter of fully albino opossums, showing that the coloring was an inherited genetic trait.
The researchers had to navigate a few hurdles to edit the opossum genome. First, they had to work out the timing of hormone injections to get the animals ready for pregnancy. The other challenge was that marsupial eggs develop a thick layer around them, called a mucoid shell, soon after fertilization. This makes it harder to inject the CRISPR treatment into the cells. In their first attempts, needles either would not penetrate the cells or would damage them so the embryos couldn’t survive, Kiyonari says.
The researchers realized that it would be a lot easier to do the injection at an earlier stage, before the coating around the egg got too tough. By changing when the lights turned off in the labs, researchers got the opossums to mate later in the evening so that the eggs would be ready to work with in the morning, about a day and a half later.
The researchers then used a tool called a piezoelectric drill, which uses electric charge to more easily penetrate the membrane. This helped them inject the cells without damaging them.
“I think it’s an incredible result,” says Richard Behringer, a geneticist at the University of Texas. “They’ve shown it can be done. Now it’s time to do the biology,” he adds.
Opossums have been used as laboratory animals since the 1970s, and researchers have att
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